in Principles and process of Biotechnology by

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Zone of inhibition is defined as an area of medium around antibiotic or any drug where there is no growth of bacteria due to its antimicrobial activity. Zone of inhibition tests also called as Kirby-Bauer test are usually performed in laboratories to detect the effectiveness of any therapeutic drug or antibiotic in inhibiting microbial growth. It is also known as Antibiotic sensitivity test where the susceptibility of bacteria to various antibiotics is determined.