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Features of cloning vector

  1. It is a double stranded DNA molecule, can able to replicate autonomously.
  2. Cloning vectors contains a cloning site also known as restriction site, to insert foreign DNA, cut by a restriction enzyme.
  3. It helps to make number of copies of a small fragment of DNA.
  4. Copy number is an essential feature of an ideal vector. It refers to the number of plasmid. Higher the copy number more ideal the vector.
  5. Origin of replication is the important property of vector design. Its presence guides the proper replication that helps in maintaining copy number high in the host.
  6. Size of a vector should be less (below 10kb). Lesser the size, more possibility of the insertion of larger gene and get easily transformed to the host.
  7. Small size decreases the probability of breakage during isolation and