The two types of ‘biological tools’ used in recombinant DNA technology are:
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Enzymes: These include Restriction enzymes (Endonucleases), which acts as molecular scissors, Ligases which are responsible for joining DNA fragments together and Reverse Transcriptase which is used for converting mRNA to cDNA.
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Vectors: They serve as a medium or vehicle to carry recombinant DNA into the host cell. The most commonly used vectors as Plasmids, Cosmids and Bacteriophages.
