Features of cloning vector
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It is a double stranded DNA molecule, can able to replicate autonomously.
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Cloning vectors contains a cloning site also known as restriction site, to insert foreign DNA, cut by a restriction enzyme.
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It helps to make number of copies of a small fragment of DNA.
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Copy number is an essential feature of an ideal vector. It refers to the number of plasmid. Higher the copy number more ideal the vector.
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Origin of replication is the important property of vector design. Its presence guides the proper replication that helps in maintaining copy number high in the host.
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Size of a vector should be less (below 10kb). Lesser the size, more possibility of the insertion of larger gene and get easily transformed to the host.
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Small size decreases the probability of breakage during isolation and
