Question

What are the requirements for PCR (Polymerase Chain Reaction)?

Answer 1

Polymerase chain reaction (PCR) is the technique to amplify specific sequences of DNA which is introduced by Kary Mullis in 1983.

Requirements for PCR is as below:

1. Target DNA: denatured at 93 - 95°C
2. PCR reaction mixture:
   1. Tris-HCL Buffer
   2. MgCl2
   3. KCl
   4. Gelatine
   5. 4 dNTPs (deoxynucleoside triphosphates) such as: dATP, dCTP, dGTP and dTTP
   6. Triton-X 100
3. Enzyme Taq (Thermus aquaticus) polymerase: thermostable
4. Primers: should be 20-30 bps long