Regeneration in photosynthesis means the regeneration of RuBP during the Calvin cycle of photosynthesis. The glyceraldehyde phosphate is converted to RuBP by utilization of one molecule of ATP. This phase is said to be regeneration phase since the RuBP is regenerated from the glyceraldehyde phosphate by phosphorylation. Regeneration of RuBP is necessary for the cycle to continue.