Chloroplasts are the green plastids which act as photosynthetic apparatus. In higher plants, they are mostly oval, spherical or discoid.
The major parts of chloroplast are:
1. Envelop:
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Each chloroplast is bounded by two smooth selectively permeable cytoplasmic membranes with an inter-membrane space which is filled with fluid.
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These membranes are composed of lipo-protein sub-units.
2. Stroma:
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It is the colourless hydrophilic ground substance (matrix) which fills the internal space of chloroplast.
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It contains various enzymes required for the synthesis of glucose during dark phase of photosynthesis.
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Matrix contains 70S ribosomes, 2-6 copies circular DNA, water and enzymes.
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The most abundant enzyme is Rubisco (Ribulose Diphosphate Carboxylase)
3. Grana:
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It is embedded in the ground substance-Stroma
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It consists of many granums connected with each other by intergranal lamellae
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Each granum is made up of stack of thylakoids.
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Thylakoids are membrane-bound flattened, disc-shaped vesicles.
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Each thylakoid consists of alternate layers of lipids and proteins embedded with quantasomes.
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The chlorophyll pigments are organized into numerous photosynthetic units called quantasomes.
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Each quantasome contains about 230 to 300 units of chlorophyll molecules.
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They are capable of trapping light energy and converting it into ATP during the light reaction of photosynthesis.
Functions:
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Chloroplasts are the site of photosynthetic reactions
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Evolution of oxygen
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Synthesis of ATP during phosphorylation
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Synthesis of glucose
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Photosensitivity in algae
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Formation of chromoplast