in Principles and process of Biotechnology by

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Genomic DNA

Plasmid DNA

1. Genomic DNA is chromosomal DNA where the genetic material is present.

1. Plasmid DNA is extra-chromosomal DNA in bacteria and some yeasts, i.e. Plasmid.

2. It is primary DNA in all living organisms.

2. It is secondary DNA.

3. It is linear in eukaryotes whereas circular in prokaryotes.

3. It is circular.

4. As it encodes genetic information, it is much larger than plasmid.

4. It is smaller.

5. Genomic DNA is organized with proteins called histones.

5. Plasmid DNA is not with histones.

6.  It contains essential genes which codes for functional and structural proteins.

6. It contains non-essential genes.

7. It can be transferred only through cell division within the same species.

7. It can be transferred through horizontal way of gene transfer between same or different species.

8. Genomic DNA cannot be used in recombinant DNA technology.

8. Plasmid DNA are used extensively in recombinant DNA technology.

9. In a normal cell only one copy of genomic DNA is present.

9. There can be multiple, identical copies of plasmids present in cell.


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