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During the zoospore formation the flagella are withdrawn and the organisms come to rest. Then the protoplasm recedes inwards from the cell wall and the contractile vacuole disappears. The protoplasm then undergoes repeated division and forms 8-16 daughter protoplasts. Then the cell wall formation occurs around each protoplast and then develops two flagella and then it becomes a parent like organism. These flagellated daughter cells are called as the zoospores. These zoospores are released by rupturing of the parental cell wall. The zoospore then grows to mature chlamydomonas.

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