Major steps in the in vitro culturing of the explants?

Question

What are the major steps in the in vitro culturing of the explants?

Answer 1

The main steps in the in vitro culturing of the explants are:

1. Sterilization of the surface of the explants

2. Nutrient medium preparation

3. Inoculation

4. Callus growth

5. Sub-culturing

6. Organogenesis

7. Direct regeneration

8. Acclimatization and transferring to the field