Difference between polymerase 1 and 3

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Lifeeasy Authors · Answer 1

No.	Feature	DNA Polymerase I	DNA Polymerase III
1.	Polymerization 5’→3’	Yes	Yes
2.	Exonuclease 3’→’5	Yes	Yes
3.	Exonuclease 5’→3’	Yes	No
4.	Use of primer single strands	Yes	No
5.	Use of nicked duplex or helix	Yes	No
6.	Molecular weight	109000 (single chain)	>250000 (heteromultimeric chain)
7.	Polypeptide chains	Two Mol. Wt. Large - 75,000 Small – 36,000	Two Mol. Wt. Large - 1,40,000 Small – 40,000
8.	No. of molecules per cell	400	10- 20
9.	Genes	pol A	pol C (dna E), dna N, dna ZX, dna Q, dna T.
10.	No. of nucleotides polymerized at 37oC molecules/ minute	Up to 1000	Up to 15000
11.	Affinity for nucleoside triphosphate (NTPs) precursors	Low	High