The main applications of the protoplasmic isolation are:
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By protoplasmic isolation and its subsequent fusion of hybrids of distant species of plants can be brought together.
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The isolated protoplasm is used for selection of mutant lines, cloning and also for studying the protoplasm.
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It is also for studying the biosynthesis of cell wall.
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The DNA ingestion can be done there by the cell can be modified.
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The regeneration of the plants from the callus which is produced as a result of the protoplast culture.
